026 Bioactivity of alpha-linolenic acid on T cell adhesion in a psoriatic skin model produced by tissue-engineering

Psoriasis is a long-lasting skin disease identified by excessive proliferation of epidermal keratinocytes. triggers an inflammatory reaction, which involuntary activates the immune system, leading to leukocyte infiltration into skin. However, supplementation diet with diverse supplements, including n-3 polyunsaturated fatty acids (n-3 PUFAs), namely alpha-linolenic acid (ALA), beneficial for psoriatic patients. exact mechanisms action ALA on immunity are still unknown. Therefore, this study was aimed evaluate bioaction lymphocyte component psoriasis, using model enriched in T cells. To do so, healthy and substitutes were produced according self-assembly technique, unsupplemented culture media or supplemented 10 uM ALA. cells isolated from whole blood donors. The medium regulated hyperproliferation abnormal cell differentiation Moreover, secreted high quantities cytokines chemokines, such as CXCL1 IL-6, while addition exogenous normalized production those cytokines. Finally, slowed down epidermis substitutes, remarkable diminished expression CD45 ICAM-1 compartment. Ultimately, our results show that exerts its anti-inflammatory decreasing biological mediators